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rneasy fibrous tissue kit  (Qiagen)
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Image Search Results


KEY RESOURCES TABLE

Journal: Neuron

Article Title: Loss of CLOCK Results in Dysfunction of Brain Circuits Underlying Focal Epilepsy

doi: 10.1016/j.neuron.2017.09.044

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Quantitative PCR in Mouse Models Total RNA was extracted from cortex of 3 control Emx-Cre; Clock +/+ and 3 Emx-Cre;Clock flox/flox mice using TRIzol reagent (Life Technologies) to homogenize tissue, and the Aurum Total RNA Mini Kit (Bio-Rad) to purify samples. cDNA was synthesized using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad).

Techniques: Diagnostic Assay, Recombinant, RNA Amplification, Reverse Transcription, SYBR Green Assay, Real-time Polymerase Chain Reaction, Microarray, shRNA, Software